This database provides median TPM values and gene ranks for all transcripts expressed in mouse renal α-intercalated cells,β-intercalated cells and principal cells ascertained from distillation of single-cell RNA-Seq data.

Brief Method: We used cell surface markers to enrich intercalated cells and principal cells from mouse kidney by FACS and carried out single-cell RNA-Seq of those cells using a microfluidic chip (Fluidigm C1 system). Single-cell cDNA libraries were constructed for paired-end sequencing and sequenced on Illumina HiSeq3000 platform. Reads were mapped to mouse Ensembl Genome by STAR and transcript abundances were calculated in the units of transcripts per million (TPM) using RSEM (https://github.com/deweylab/RSEM). Unsupervised cluster analysis revealed five different cell types, namely α-intercalated cells, β-intercalated cells, principal cell, proximal tubule cells and non-epithelial cells. The data presented below shows the median TPM values of α-intercalated cells (n=87), β-intercalated cells (n=23) and principal cells (n=74).

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Reference: paper under review

This website was created by Lihe Chen and Mark Knepper. Contact us with questions or comments: knep@helix.nih.gov.

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Sort database by: Choose from Menu Gene Symbol Alpha IC Rank Alpha IC Selective Transcripts Beta IC Rank Beta IC Selective Transcripts IC Selective Transcripts PC Rank PC Selective Transcripts Transcription Factors Only Transporters and Channels Only Protein Kinases Only 7-Membrane Spanning Receptors Only Metabolic Enzymes Only E3 Ubiquitin Ligases Only Mean TPM Null Rate